Linear regression plots for the number of adherent SS RBC/100 μm2 according to the venular diameter in preparations treated with 7E3 F(ab′)2 (or a control antibody) and PAF.
In the presence of OC125 F(ab′)2 (control antibody), PAF caused a pronounced adhesion of SS RBC that was inversely correlated with the vessel diameter (r = −.72, P < .00001). In contrast, 7E3 resulted in marked inhibition of SS RBC adhesion in venules, particularly in small-diameter venules (the sites of maximal adhesion and frequent blockage) that resulted in a lack of correlation between adhered cells and the vessel diameter (r = −.04, P > .78). A comparison of the two regression lines revealed pronounced differences in intercepts (OC125/PAF/SS 0.54 ± 0.04, 7E3/PAF/SS 0.03 ± 0.01; P < .0001).
