TNF production by HLA-A1 HY after stimulation with HeLa transfected with Y-specific cDNA.

HeLa cells were cotransfected with each Y-specific cDNA and HLA-A1 cDNA (black bars), or HLA-A1 stably transfected HeLa cells were transfected with each Y-specific cDNA only (hatched bars). Forty-eight hours after transfection, HLA-A1 HY was added. The culture supernatants were harvested 1 day later and were tested for TNF production.