Minigenes encode epitope recognized by HLA-A1 HY.

HeLa cells were cotransfected with DFFRY2 or minigene cDNA and HLA-A1 cDNA (black bars), or HLA-A1 stably transfected HeLa cells were transfected with DFFRY2 cDNA only (hatched bars). As a control, HeLa cells were transfected with DFFRY2 or minigene cDNA only (white bars). Forty-eight hours after transfection, HLA-A1 HY was added. The culture supernatants were harvested 1 day later and were tested for TNF production.