After ⁵¹Cr-labeling, the female recipient HLA-A1–positive EBV-LCL cells were pulsed with the DFFRY-derived peptide IVDCLTEMY and female HLA-B7–positive EBV-LCL cells with the SMCY-derived peptide SPSVDKARAEL at various concentrations.

HLA-A1 HY and HLA-B7 HY were added at an effector-to-target ratio of 10, and ⁵¹Cr release was measured after 4 hours.