Canine–human chimeras of GP Ib.

(A) Protocol for generating canine–human GP Ibα chimeras from wild-type canine GP Ibα cDNA (solid box) and human GP Ibα cDNA (open box) by a blunt-end ligation method (see “Materials and Methods” for details). (B) Canine–human chimeras of the GP Ibα N-terminal 282 residues. Residue numbers at domain boundaries correspond to the human GP Ibα sequence.21 Chimeras corresponding to boundaries between leucine-rich repeats 4/5 (C128) and 6/7 (C176) were not included in the study. (C) Human–canine chimeras of GP Ibα corresponding to boundaries between leucine-rich repeats.