Binding of chimera-expressing CHO cells to vWf under flow.

Representative results for binding of CHO βIX cells and CHO βIX cells expressing wild-type GP Ibα (αβIX) or the canine–human chimeras C35, C200, and C282. Upper panels show the cells in PBS buffer (5 × 10⁶/mL) in the flow chamber before the application of flow. Lower panels show overlayed sequential images snapped at 30 frames/s during a 4-minute period of perfusion in the direction of the arrow at 10 dynes/cm² over a glass surface coated with 50 μg/mL vWf. Results are representative of 3 to 5 experimental runs performed using different populations of cells. Arrowheads show points of adhesion for translocating cells expressing chimera C200.