Intracellular RANTES expression in CBL and ABL.

CBL and ABL were either untreated or stimulated with PMA (5 ng/mL) for 12 hours, after which staining for RANTES and the cell-surface markers CD4, CD8, CD45RA, and CD45RO was performed as previously described. (A) Flow cytometric RANTES expression in CBL and ABL. The shaded histograms represent control staining with irrelevant mAb, and the open histograms represent cells staining positive for RANTES. Percentages of cells staining positive for RANTES are shown. (B) RANTES expression in lymphocyte subsets (2-color flow cytometry). Results (mean ± SEM) are shown as percentages of cells in each subset expressing RANTES and as relative fluorescence intensity (RFI = mean fluorescence intensity of sample minus mean fluorescence intensity of control/mean fluorescence intensity of control). The results are representative of 6 pairs of cord and adult blood specimens. *Indicates CD8 versus CD4, P < .05. **Indicates CD45RO versus CD45RA, P < .05.