Effects of ATP on the actin polymerization in eosinophils.

(A) Cells were stimulated with ATP in 5 concentrations: 1 mmol/L, 100 μmol/L, 10 μmol/L, 1 μmol/L, and 0.1 μmol/L. The relative f-actin content was determined at the indicated time points by flow cytometry. Data are means ± SEM (standard error of the mean; n = 8). (B) Representative histogram (25-second time point) of eosinophils stimulated with control buffer (➁) compared with eosinophils stimulated with 1 mmol/L of ATP (➀). Representative data of 1 experiment are shown. The experiments were repeated 8 times with identical results.