Fig. 3.
![Fig. 3. Effect of the inhibition of APN activity on cultured cell growth. / (A) Sorted CD13hi and CD13lo cells were incubated overnight in the presence of 3[H]dThd, with or without 10−4 mol/L arphamenine B (Arpha.B) or actinonin; data are from 1 experiment of 2. (B) Bulk culture day 5 or day 7 cells were treated in the same manner as in A; data are presented as mean [3H]dThd incorporation ± SD (n = 6); statistical significance of differences: P < .001 for actinonin vs control and vs arphamenine B. (C) Culture day 7 cells were incubated overnight with CD13 mAb F23, WM15 or TÜK1, or with isotype control mAb IgG2a for F23 and IgG1 for WM15 and TÜK1 (all at 50 μg/mL) in the presence of [3H]dThd; data are from 1 experiment of 2.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/95/2/10.1182_blood.v95.2.453/6/bloo00223003x.jpeg?Expires=1735541448&Signature=VndfKBze2CViUN9ha-BGzskmMNEoTD5vQbCwjXcptLdKDUYQxE-JmfbrnmjWW-BadFiYOKzh9EW0JL98QF5JywiDH10moKB44p8P4zhCl9YYUQ9GmFkeYQIoRi~7iFIlQMWHrX8NESi-vkNzUBh3-5-FDFuMAeeOU5J2GesK8bGiPVqCVdXiaq1GdvedVm1ctE4qUNlZZEHBT47CNcNA3E5vOFq51jy~bBcaxakoeDYOuukpGl1WWR2rYVFRCaXhiv69nmCLXpG19k-MQ-A1benI-tl4975BpBcMGebxy8AaCksK9eFnUlUEvibmBEZJ-HB2HsFr10ihhoibEE7bOQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effect of the inhibition of APN activity on cultured cell growth.
(A) Sorted CD13hi and CD13lo cells were incubated overnight in the presence of 3[H]dThd, with or without 10−4 mol/L arphamenine B (Arpha.B) or actinonin; data are from 1 experiment of 2. (B) Bulk culture day 5 or day 7 cells were treated in the same manner as in A; data are presented as mean [3H]dThd incorporation ± SD (n = 6); statistical significance of differences: P < .001 for actinonin vs control and vs arphamenine B. (C) Culture day 7 cells were incubated overnight with CD13 mAb F23, WM15 or TÜK1, or with isotype control mAb IgG2a for F23 and IgG1 for WM15 and TÜK1 (all at 50 μg/mL) in the presence of [3H]dThd; data are from 1 experiment of 2.
