Fig. 4.
![Fig. 4. Inhibition of APN activity on CEM and U937 cells. / (A) Assay of APN activity of CEM or U937 cells incubated for 6 hours with different concentrations of arphamenine B (CEM(arpha), U937(arpha)) or actinonin (CEM(act), U937(act)); mean percentages ± SD (n = 2) of the APN activity of treated cells relative to control cells without inhibitor. (B) CEM or U937 cells were cultured overnight in the presence of [3H]dThd, with or without arphamenine B or actinonin at the indicated concentrations; each bar corresponds to the mean (+ SD) of 3 to 8 independent determinations; the significance of differences between percentages of incorporated cpm and 100% control values was assessed by the 1 sample t test: ***P < .001.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/95/2/10.1182_blood.v95.2.453/6/bloo00223004x.jpeg?Expires=1735541382&Signature=INgSaoraCP2r2syKrDTWkz0kIOhFfXt6jgDWf9DxfaeSU~3MQarbQS6js8ptdkJWVJJ4kC20M3djRw8GQW~~Ap4y82gOcrmTGryv7dkFoJDnPW9hwy7OTfdg4h~lOoSLJAm-SbfNnbO1LDMOGUBOEKDJnhHbjYHo14J2tGYQ-TXGW5gjohvj4LCv3fDTQnKJbr3UAIRGDqf7veoHypwQkwua60GJqKZQIOhjEhVm5pz~1LlKy-7SdA7bHkIOwnhLfVvmShIQ4n3OJoNTKG7dQECQWF0vw-nEwMYiMwWPfXVf~-hWi0jqdg5L2LLOWHvlxQFkqPPY50FkMQVZyC8Y~Q__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Inhibition of APN activity on CEM and U937 cells.
(A) Assay of APN activity of CEM or U937 cells incubated for 6 hours with different concentrations of arphamenine B (CEM(arpha), U937(arpha)) or actinonin (CEM(act), U937(act)); mean percentages ± SD (n = 2) of the APN activity of treated cells relative to control cells without inhibitor. (B) CEM or U937 cells were cultured overnight in the presence of [3H]dThd, with or without arphamenine B or actinonin at the indicated concentrations; each bar corresponds to the mean (+ SD) of 3 to 8 independent determinations; the significance of differences between percentages of incorporated cpm and 100% control values was assessed by the 1 sample t test: ***P < .001.
