Effect of the inhibition of APN activity on the cell cycle and the induction of apoptosis.
Culture day 7 cells were incubated overnight with or without 10−4 mol/L arphamenine B or actinonin before being examined. (A) DNA content was determined by flow cytometry after ethanol fixation, permeabilization and PI staining: percentages of cells in the G0/G1 or S/G2-M phase, assessed relative to viable cell numbers, are indicated; data are from 1 representative experiment out of 7. (B) Nuclear morphology was visualized by ultraviolet light microscopy after Hoechst 33 342 staining; the arrow shows a typical apoptotic cell; data are from 1 experiment of 3. (C) Surface expression of inner membrane PS was evaluated by flow cytometry after Annexin-V staining: the percentage of cells with increased PS expression is indicated; data are from 1 experiment of 4.
