GCKR involvement in CrkL-induced SAPK activation in K562 cells.

K562 cells were transfected with contructs directing the expression of CrkL (lanes 1 to 3), antisense GCKR (lane 1), kinase inactive GCKR (lane 2), or wild-type GCKR (lane 4). Levels of phosphorylated SAPK were detected with an antibody specific for pSAPK. Levels of SAPK, GCKR, and CrkL in the cell lysates were detected by immunoblotting with SAPK, GCKR, and CrkL-specific antibodies. This experiment was performed twice with similar results.