Loxosceles venom components incorporate into E and cause loss of expression of glycophorins.

E were treated with buffer (C), L. intermedia venom, P1, P2, or P3 toxins (10 μg/mL each), and analyzed for the expression of GPA and GPC by flow cytometry using the antibodies Bric256 (GPA) and Bric4 and Bric10 (GPC). The ability of the toxins P1, P2, and P3 to insert into the E surface was analyzed using a monospecific polyclonal rabbit serum (F35) against the toxins. Results are representative of 3 different experiments and are expressed as mean of triplicates ± SD.