P2 toxin induces cleavage of GPC from nucleated cells.

K562, U937, and Jurkat cells were incubated for 30 minutes with buffer (thick line) or with 10 μg of P2 toxin (thin line), washed and stained for flow cytometry using the monoclonal antibody Bric4 against GPC. Background fluorescence (shaded histogram). Results are representative for 2 different experiments.