Thymic lobes stained for DEC-205 expression.

WT or PU.1^−/−embryos or cultured thymic lobes were fixed, and cryosections were prepared. Individual sections were stained with αDEC-205 antibodies (Figure 2). E14.5 and E16.5 indicate the gestational age of the embryo. FTOC indicates E16.5 thymic lobes that have been placed in organ culture for an additional 10 days prior to sectioning.