t-SNAREs in the platelet.

Sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting analyses were used to demonstrate the presence of SNAP-23 (A) and syntaxins 2, 4, and 7 (B) in platelets. One hundred micrograms of whole platelet lysate was separated by SDS-PAGE and then transferred to nitrocellulose. The resulting blots were probed with anti–syntaxin 2 (syntaxin 2), anti–syntaxin 4 (syntaxin 4), and anti–syntaxin 7 (syntaxin 7), and the immunodecorated proteins were detected by ECL. For A, the anti–SNAP-23 antibody was preincubated with 500 μg of either recombinant SNAP-23 or SNAP-25, as indicated.