Fig. 6.
![Fig. 6. Anti–SNAP-23 antibody inhibits [3H]5-HT release. / (A) The permeabilized platelet exocytosis was performed in the presence of increasing amounts of Fab23 (0-0.16 mg/mL). After 5 minutes of Ca++ stimulation (10 μmol/L), [3H]5-HT release was measured (n = 3). (B) Secretion triggered by 10 μmol/L Ca++ was analyzed in the presence of Fab23 (0.12 mg/mL), Fab23 (0.12 mg/mL) preincubated with His6–SNAP-23 (0.45 mg/mL), His6–SNAP-23 (0.45 mg/mL), human SNAP-23 C-terminal peptide (0.15 mg/mL), and rabbit IgG purified from preimmune sera (0.15 mg/mL) (n = 6).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/95/3/10.1182_blood.v95.3.921.003k17_921_929/6/bloo00317006x.jpeg?Expires=1735514635&Signature=Rixl1wqdpm3oEKbI0zaF1xNPtzhLXBLmER27pUq2iI5rukbE9MuIJRbMhyDq04aQvPtnhz80AEZe~ENMcDfY99GT1Z3OWuBYK3wXaNtKlzSmHfdML1xTy2oJaXV8-sWhYrTEyTDHyfFkWOdikODP1ohxhsQskt0Gu6drwQaBmJ8~cBOvsu3lOYLhkn~FSKzWn3nooTgxA7-9kXjqPOvndpdwZc7auzp9R-V7ma7uH10n~4CE1~f8Wkhz9sb6mnpqjMDwXr-MygUTHLnDVkFVqY7C4RGq3KA~Sm~sv7gSDA5A9ZbREwGnnMGwWFNxP1L3ZMZ3tiIPbQ0Vkq0MqtWK3w__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Anti–SNAP-23 antibody inhibits [3H]5-HT release.
(A) The permeabilized platelet exocytosis was performed in the presence of increasing amounts of Fab23 (0-0.16 mg/mL). After 5 minutes of Ca++ stimulation (10 μmol/L), [3H]5-HT release was measured (n = 3). (B) Secretion triggered by 10 μmol/L Ca++ was analyzed in the presence of Fab23 (0.12 mg/mL), Fab23 (0.12 mg/mL) preincubated with His6–SNAP-23 (0.45 mg/mL), His6–SNAP-23 (0.45 mg/mL), human SNAP-23 C-terminal peptide (0.15 mg/mL), and rabbit IgG purified from preimmune sera (0.15 mg/mL) (n = 6).
