SNAP-23 and syntaxin 2 can form a complex.

Fifty micrograms of Triton X-100–solubilized platelet extracts from resting and thrombin-activated (1 U/mL for 5 minutes) platelets was subjected to immunoprecipitation (IP) with ab23 coupled to protein G beads. The precipitated material (P) and the unbound supernatant material (S) were analyzed by Western blotting with ab23 and anti–syntaxin 2 antibody. The immunodecorated proteins were detected by ECL as described earlier. Hexosaminidase release was measured to confirm the activation of platelets.