Vector-specific PCR.

(A) PCR was performed with the use of DNA isolated from peripheral blood samples taken from primary recipients before and after MMC administration. The standard curve was generated with the use of serial dilution of a known vector-copy cell line, 52-19. (B) Methylcellulose cultures were performed with the use of bone marrow isolated from animals receiving FANCC-transduced cells. Day-15 bone marrow colonies were enumerated and isolated for PCR analysis. (C) PCR was performed with the use of DNA isolated from peripheral blood samples taken from secondary recipients before and after MMC administration. Positive control DNA was derived from cell line 52-19 and negative control DNA was isolated from cell line 17.