Rolling flux of CD34⁺ and CD34⁻ FL cells on P- and L-selectin at a shear rate of 210 s⁻¹.

Cells were subjected to wall shear rates from 487 s⁻¹to 77 s⁻¹ on P- or L-selectin chimeras adsorbed at 2 μg/mL on silanated glass. Rolling flux, rolling velocity, and rolling concentration was determined at 10 shear rates on P- and L-selectin, but only the rolling flux at a representative shear rate of 210 s⁻¹ is shown. In control experiments, chimera-coated slides were incubated with mAb. (A) Rolling flux on P-selectin. Control experiment used the anti-P selectin (CD62P) mAb, G1. (B) Rolling flux on L-selectin. Control experiment used the anti-L selectin (CD62L) mAb, DREG-56. Data presented are from single experiments.