CD34+ and CD34− ABM cells rolling on E-selectin.
Cells were subjected to wall shear rates from 487 s−1to 77 s−1 on E-selectin chimera adsorbed at 2 μg/mL on silanated glass. (A) Rolling flux. Rolling flux was determined at 10 shear rates for each experiment. (B) Rolling velocity. Rolling velocities were determined for every rolling cell at each shear rate for which there were 2 or more rolling cells and then averaged at each shear rate for each experiment. At higher shear rates for which there were < 2 rolling cells, mean rolling velocity is not presented. (C) Rolling concentration. Rolling concentration ( = rolling flux/mean rolling velocity) was determined at 10 shear rates for each experiment. Each data point represents results from 2 independent experiments. Data are presented as mean ± SEM.
