Protein expression of different PKC isoforms in freshly isolated CD34⁺ progenitor cells from bone marrow.

B cells (BC) and the glioma cell lines BT4c and U87GM were included as positive controls. The CD34⁺ cells were selected as described in “Materials and Methods,” and cytosolic proteins were prepared and analyzed by Western blotting. A representative of 3 separate experiments is shown. Note that for B cells and CD34⁺ cells, the bands below 80 kd for PKC-α, PKC-β2, and PKC-ε were possible degradation products resulting from activation.