American Society of Hematology

Fig. 3.

$Fig. 3. Protein expression of PKC- and PKC-β2 in cytosolic and membrane fractions during EPO and SCF stimulation of CD34+ cells for various times. / CD34+ cells were cultured in the presence of EPO (5 U/mL) and SCF (50 ng/mL) for various times before the preparation of cytosolic and membrane proteins. Protein expressions of PKC-α, PKC-β2, and Bcl-xL were determined by Western blotting. A representative of 3 separate experiments is shown. C, cytosolic fractions; M, membrane fractions; d, days.$

Protein expression of PKC- and PKC-β2 in cytosolic and membrane fractions during EPO and SCF stimulation of CD34⁺ cells for various times.

CD34⁺ cells were cultured in the presence of EPO (5 U/mL) and SCF (50 ng/mL) for various times before the preparation of cytosolic and membrane proteins. Protein expressions of PKC-α, PKC-β2, and Bcl-x_L were determined by Western blotting. A representative of 3 separate experiments is shown. C, cytosolic fractions; M, membrane fractions; d, days.

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