American Society of Hematology

Fig. 4.

$Fig. 4. Protein expression of PKC-, PKC-β2, and PKC-ɛ in cytosolic and nuclear fraction of CD34+ cells during EPO stimulation for various times. / CD34+ cells were cultured in medium alone for 24 hours before the addition of EPO (5 U/mL) and then were incubated for various times before cytosolic and nuclear proteins were prepared. The protein expression of PKC-α, PKC-β2, and PKC-ε was determined by Western blotting. (A) A representative of 5 separate experiments is shown. *, not performed. (B) Optical density of PKC-α determined by densitometric imaging of hyperfilms is shown. Mean ± SEM of 5 separate experiments (*P = .028, n = 5). (C) Optical density of PKC-β2 determined by densitometric imaging of hyperfilms is shown. Mean ± SEM of 5 separate experiments (*P = .047, n = 5). (D) Optical density of PKC-ε determined by densitometric imaging of hyperfilms is shown. Mean ± SEM of 3 separate experiments.$

Protein expression of PKC-, PKC-β2, and PKC-ɛ in cytosolic and nuclear fraction of CD34⁺ cells during EPO stimulation for various times.

CD34⁺ cells were cultured in medium alone for 24 hours before the addition of EPO (5 U/mL) and then were incubated for various times before cytosolic and nuclear proteins were prepared. The protein expression of PKC-α, PKC-β2, and PKC-ε was determined by Western blotting. (A) A representative of 5 separate experiments is shown. *, not performed. (B) Optical density of PKC-α determined by densitometric imaging of hyperfilms is shown. Mean ± SEM of 5 separate experiments (*P = .028, n = 5). (C) Optical density of PKC-β2 determined by densitometric imaging of hyperfilms is shown. Mean ± SEM of 5 separate experiments (*P = .047, n = 5). (D) Optical density of PKC-ε determined by densitometric imaging of hyperfilms is shown. Mean ± SEM of 3 separate experiments.

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