Fig. 3.
Fig. 3. Induction of p53 and p21 transcription and expression by CD40 activation of RPMI 8226 and HS Sultan MM cell lines. / RPMI 8226 (A, B) and HS Sultan (C, D) cells were cultured in media and with formalin-fixed NIH3T3/CD40LT, NIH3T3/wt, or NIH3T3/vt at 37°C or 30°C for 72 hours. p53 and p21 mRNA expression (A, C) was assayed using RPA; p53 and p21 protein expression (B, D) was assayed using Western blotting. β-Actin probe and DM 1A anti-tubulin mAb served as controls for RPA and Western blotting, respectively. The relative intensity of expression was assessed using imaging densitometry and was normalized against expression in control cells cultured in media without NIH3T3/CD40LT. Relevant data of CD40-triggered tumor cells are presented as normalized mRNA or protein expression.

Induction of p53 and p21 transcription and expression by CD40 activation of RPMI 8226 and HS Sultan MM cell lines.

RPMI 8226 (A, B) and HS Sultan (C, D) cells were cultured in media and with formalin-fixed NIH3T3/CD40LT, NIH3T3/wt, or NIH3T3/vt at 37°C or 30°C for 72 hours. p53 and p21 mRNA expression (A, C) was assayed using RPA; p53 and p21 protein expression (B, D) was assayed using Western blotting. β-Actin probe and DM 1A anti-tubulin mAb served as controls for RPA and Western blotting, respectively. The relative intensity of expression was assessed using imaging densitometry and was normalized against expression in control cells cultured in media without NIH3T3/CD40LT. Relevant data of CD40-triggered tumor cells are presented as normalized mRNA or protein expression.

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