Fig. 4.
Effect of temperature on viability of CD40-activated RPMI 8226 and HS Sultan MM cell lines.
RPMI 8226 (A, B) and HS Sultan (C, D) cells (0.7 × 106initiating cells/well) were cultured in media (○) and with formalin-fixed NIH3T3/CD40LT (•), NIH3T3/wt (□), or NIH3T3/vt (▪) for up to 144 hours. Tumor cells were cultured at 37°C (A, C) or 30°C (B, D), and viable cell density was assessed at 0, 2, 8, 16, 24, 36, 48, 72, 96, 120, and 144 hours using trypan blue exclusion. Experiments were performed in triplicate, and results are expressed as mean ± SEM.
