Fig. 6.
Effect of temperature on cell cycle profile of CD40-activated RPMI 8226 and HS Sultan MM cell lines.
RPMI 8226 (A) and HS Sultan (B) cells were cultured in media and with formalin-fixed NIH3T3/CD40LT, NIH3T3/wt, or NIH3T3/vt at 37°C or 30°C for up to 144 hours. The cell cycle profile was assessed using PI staining and flow cytometric analysis (0.5 × 106cells/sample) at 0 hour and at culture conditions in which maximum cell cycle effects were noted at 24 hours for cultures at 37°C and at 144 hours for cultures at 30°C.
