Fig. 4.
Sequence-specific cleavage ofBCR-ABLp190 mRNA by M1-p190-GS RNA in Ba/F3+p190 cells transfected with the MFG-M1-p190-RNA vector.
Total RNA was isolated from Ba/F3 + p190 cells (lane 1), Ba/F3 + p190 cells transfected with the MFG-M1-p190-GS expression vector (lane 2), and Ba/F3 + p190 cells transfected with the MFG-M1-p210-GS expression vector (lane 3). Cellular RNA was hybridized to a human ABL probe (A), to a mouse β-actin cDNA (B), to a human Bcl-2 probe (C), and to a 3′-untranslated MFG probe (D). Autoradiography was for 9 hours at −70°C.