Fig. 5.
Fig. 5. Specific suppression ofBCR-ABLp210 mRNA transcripts in Ba/F3 + p210 cells transfected with the MFG-M1-p210-RNA vector. / Total RNA was isolated from Ba/F3 + p210 cells (lane 1), Ba/F3 + p210 cells transfected with the MFG-M1-p190-GS expression vector (lane 2), and Ba/F3 + p210 cells transfected with the MFG-M1-p210-GS expression vector (lane 3). Cellular RNA was hybridized to a human ABL probe (A), to a mouse β-actin cDNA (B), to a human Bcl-2 probe (C), and to a 3′-untranslated MFG probe (D). Autoradiography was for 9 hours at −70°C.

Specific suppression ofBCR-ABLp210 mRNA transcripts in Ba/F3 + p210 cells transfected with the MFG-M1-p210-RNA vector.

Total RNA was isolated from Ba/F3 + p210 cells (lane 1), Ba/F3 + p210 cells transfected with the MFG-M1-p190-GS expression vector (lane 2), and Ba/F3 + p210 cells transfected with the MFG-M1-p210-GS expression vector (lane 3). Cellular RNA was hybridized to a human ABL probe (A), to a mouse β-actin cDNA (B), to a human Bcl-2 probe (C), and to a 3′-untranslated MFG probe (D). Autoradiography was for 9 hours at −70°C.

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