Fig. 6.
Fig. 6. Comparison of suppression ofBCR-ABLp190 mRNA transcripts. / Direct comparison of suppression ofBCR-ABLp190 mRNA transcripts in Ba/F3 + p190 cells transfected either with the MFG-M1-p190-RNA vector or with the same vector carrying only theBCR-ABLp190 antisense sequences. Total RNA was isolated from Ba/F3 + p190 cells transfected with the MFG-M1-p190-GS expression vector (lanes 1-3), control Ba/F3 + p190 cells (lane 4), and Ba/F3 + p190 cells transfected with the MFG-p190-antisense expression vector32 (lane 5). Cellular RNA was hybridized to a human ABL probe. RNA integrity was demonstrated by hybridizing the same filter with a mouse β-actin cDNA probe. Autoradiography was for 9 hours at −70°C.

Comparison of suppression ofBCR-ABLp190 mRNA transcripts.

Direct comparison of suppression ofBCR-ABLp190 mRNA transcripts in Ba/F3 + p190 cells transfected either with the MFG-M1-p190-RNA vector or with the same vector carrying only theBCR-ABLp190 antisense sequences. Total RNA was isolated from Ba/F3 + p190 cells transfected with the MFG-M1-p190-GS expression vector (lanes 1-3), control Ba/F3 + p190 cells (lane 4), and Ba/F3 + p190 cells transfected with the MFG-p190-antisense expression vector32 (lane 5). Cellular RNA was hybridized to a human ABL probe. RNA integrity was demonstrated by hybridizing the same filter with a mouse β-actin cDNA probe. Autoradiography was for 9 hours at −70°C.

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