Fig. 5.
Fig. 5. Family study of the Hpdel allele in 2 families of anhaptoglobinemic patients with anti-haptoglobin antibody. / Simultaneous PCR amplification of the junction region of theHpdel allele and of exon 1 of theHp was carried out. PCR products underwent electrophoresis in a 1.2% agarose gel and were stained by ethidium bromide. Lanes 1, 2, and 3 are SN and 2 children of SN. Lanes 4, 5, 6, and 7 are NK, father, mother, and brother of NK. Lanes 8 and 9 are control individuals. Lane 10 is negative control. The size of the PCR product is indicated.TaqI-digested φ × 174 (M1) andHindIII-digested λ phage (M2) were used as molecular size markers.

Family study of the Hpdel allele in 2 families of anhaptoglobinemic patients with anti-haptoglobin antibody.

Simultaneous PCR amplification of the junction region of theHpdel allele and of exon 1 of theHp was carried out. PCR products underwent electrophoresis in a 1.2% agarose gel and were stained by ethidium bromide. Lanes 1, 2, and 3 are SN and 2 children of SN. Lanes 4, 5, 6, and 7 are NK, father, mother, and brother of NK. Lanes 8 and 9 are control individuals. Lane 10 is negative control. The size of the PCR product is indicated.TaqI-digested φ × 174 (M1) andHindIII-digested λ phage (M2) were used as molecular size markers.

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