Fig. 4.
Phagocytosis of rabbit IgG-opsonized SRBC and FcγR-specific, mAb-coated SRBC by neutrophils from donor 17.
Neutrophils isolated from control (closed bar) or D17 (hatched bar) were analyzed for the phagocytosis of EA (A) or Fab fragments of indicated mAb-coated SRBC (B) as described in “Materials and Methods.” The pseudoperoxidase activity of the hemoglobin from the ingested E was assayed using o-tolidine, and the color formed was measured at 405 nm in an enzyme-linked immunosorbent assay reader.