Effect of fMLP treatment of neutrophils on rosette formation with EA.

Neutrophils (1 × 10⁷/mL) from control and donor 17 were incubated with RPMI 1640 medium without serum in the absence or presence of fMLP (1 μmol/L, 10 μmol/L) for 30 minutes at 37°C. After washing the cells in RPMI 1640/2% IgG free fetal bovine serum, the rosette formation of neutrophils from donor 17 (A) and control (B) with E was performed as described in “Materials and Methods.” Experiments were performed in duplicate.