Apoptosis in CD38-positive B-CLL cells exposed to Gaμ-ab or Gaδ-ab.

(A) B-CLL cells were cultured in the presence of Gaμ-ab, Gaδ-ab, or NGI at the concentration of 10 μg/mL. After 18 hours, apoptotic cells in the cultures were measured by PI staining of permeabilized cells. Data represent the mean ± SD of the values obtained in the 10 patients with B-CLL (A-L). (B) B-CLL cells from patient G were incubated with the indicated concentrations of Gaμ-ab (▪), Gaδ-ab (▴), or NGI (○) for 18 hours, after which apoptosis was determined as above. (C) B-CLL cells from patient D were cultured in the presence of Gaμ-ab, Gaδ-ab, or NGI for the indicated time. At the end of the cultures, cells were double stained in suspension with Annexin-V FITC (to detect apoptotic cells) and PI (to detect late apoptotic or nonviable cells) and then analyzed by flow cytometry. Data in B and C represent 1 typical experiment of the 6 performed (patients B, C, G, D, F, I).