![Fig. 4. Ability of Gaμ-ab and Gaδ-ab to activate the signal transducing pathway in CD38-positive B-CLL cells. / (A) B-CLL cells (patient G) were stimulated with NGI, Gaμ-ab, or Gaδ-ab at the optimal concentration (10 μg/mL) for the indicated time. Cell lysates (20 μg/lane) were separated on 10% reducing SDS-PAGE gels and analyzed by immunoblotting with the antiphosphotyrosine mAb, 4G10. Molecular weight in kilodalton is indicated on the right. (B) B-CLL cells (patient H) were stimulated for 3 or 5 minutes with the indicated stimuli. Cell lysates were immunoprecipitated with antiphosphotyrosine mAb (4 G10). The immunoprecipitates were separated on 10% reducing SDS-PAGE gels and probed with an anti-syk mAb (top) or anti-PLCγ1 mAb (bottom). (C) Fura2/AM loaded B-CLL cells were stimulated with Gaμ-ab or Gaδ-ab and studied for [Ca++]i mobilization. Means ± SD of the experiments on the 10 different patients with B-CLL (patients A-L) examined. Panels on the right show the typical profiles of [Ca++]i response to the indicated stimuli (patient A).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/95/4/10.1182_blood.v95.4.1199.004k21_1199_1206/6/bloo00421004cx.jpeg?Expires=1735502924&Signature=daIVguVohfiZbvUINvT-sq4KBSXCHojs6EQvuK78GQay0u1vRzGoKbEd2caSLg-odzzOVXbCCnl6bx~-eVLrbBAH4tC1QSx9jPK97HwzYzWIuRPz~IBsD7hXQUzFrNmq29-ZgoHzCBkz7yw09twkr5gnHaMBgfllfK3ZB32djKC~YmujKmdeAtcp4ltJAjMWEnDNmhuYUyrawnETCN6qEKqvPFk5QZG7~zGLgr68btn3JgF1IRFu5QI0QnFjsd-427u5RfVZjdrMiDwyvtb0ldEst30Duv2tDv9u7w9wH0uoXiLOBVFfFKATySq1S-QpPL-Y13y9M4C0ayx1H7nQZQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Ability of Gaμ-ab and Gaδ-ab to activate the signal transducing pathway in CD38-positive B-CLL cells.
(A) B-CLL cells (patient G) were stimulated with NGI, Gaμ-ab, or Gaδ-ab at the optimal concentration (10 μg/mL) for the indicated time. Cell lysates (20 μg/lane) were separated on 10% reducing SDS-PAGE gels and analyzed by immunoblotting with the antiphosphotyrosine mAb, 4G10. Molecular weight in kilodalton is indicated on the right. (B) B-CLL cells (patient H) were stimulated for 3 or 5 minutes with the indicated stimuli. Cell lysates were immunoprecipitated with antiphosphotyrosine mAb (4 G10). The immunoprecipitates were separated on 10% reducing SDS-PAGE gels and probed with an anti-syk mAb (top) or anti-PLCγ1 mAb (bottom). (C) Fura2/AM loaded B-CLL cells were stimulated with Gaμ-ab or Gaδ-ab and studied for [Ca++]i mobilization. Means ± SD of the experiments on the 10 different patients with B-CLL (patients A-L) examined. Panels on the right show the typical profiles of [Ca++]i response to the indicated stimuli (patient A).
