Megakaryocyte-specific expression of the GPIIb promoter containing mutations in the putative negative regulatory element.

Hematopoietic cells were generated from ES cells by the addition of TPO (A, E), EPO (B, F for primitive embryonic erythrocytes and C, G for definitive adult erythrocytes), and M-CSF (D, H) for megakaryocytes, erythroid cells, and macrophages, respectively. The wild-type and mutant GPIIb promoter constructs were transfected and analyzed as described in Figure 2. The data from the wild-type (−598/+33) promoter (A-D) and the mutated promoter in which the nucleotides between −148 and −89 (E-H) were deleted are shown. Promoters lacking −118 to −89 and −148 to −59 show similar results.