Mouse TLR2 and TLR4 gene expressions in mouse T cells.

(A) Mouse T cells purified from thymuses and spleens were either unstimulated or stimulated with immobilized anti-CD3ε mAb for 2 hours. Total RNAs were extracted, and mTLR2 and mTLR4expressions were analyzed by semiquantitative RT-PCR. (B) S49.1 and EL4 cells were either unstimulated or stimulated with 100 ng/mL PMA plus 1 μg/mL ionomycin for 2 hours. We isolated 5 μg mRNA from the cells and analyzed them by Northern blot analysis using mTLR2 and mTLR4 cDNA probes. (C) CTLL-2 cells were starved overnight without factors and either left unstimulated or stimulated with 10 ng/mL mIL-2 or 10 ng/mL hIL-15 for the indicated time.