Involvement of MAP kinase pathways in mTLR2 induction in S49.1 cells.
(A) S49.1 cells were either untreated or treated with PMA plus ionomycin for 2 hours with or without the pretreatment of 10 μmol/L PD98 059 or SB208 530 for 30 minutes. S49.1 cells were also treated with UV irradiation (40 J/m2) and left for 1 hour at 37°C. In some experiments, S49.1 cells constitutively expressing a dominant negative form of JNK1 or H-Ras were treated with PMA plus ionomycin for 2 hours. Total RNAs were extracted, and the expression ofmTLR2, mTLR4, and β-actin was examined by semiquantitative RT-PCR. (B) S49.1 cells were pretreated with a series of concentrations of PD98 059 or SB208 530 for 30 minutes followed by treatment with PMA plus ionomycin for 2 hours. Total RNAs were extracted, and the expressions of mTLR2, mTLR4, andβ-actin were examined by semiquantitative RT-PCR. (C) Total RNAs were extracted from S49.1 cells transfected with pEGFP-C1 or pEGFP-MEK1A. Expression of mTLR2 and β-actin was examined by semiquantitative RT-PCR.
