Expression of MEFV mRNA in different populations of peripheral blood cells.

Total RNAs were isolated from the indicated subsets of leukocytes and subjected to RT-PCR using specific oligonucleotides for MEFV, lactoferrin, and β-actin. Amplification occurred for 30, 25, and 22 cycles, respectively. PCR fragments were separated by agarose gel electrophoresis, transferred to a nylon membrane, and hybridized with a gene-specific internal oligonucleotide labeled with DIG. (PMN = polymorphonuclear cells (neutrophils); Monoc. = monocytes; M = DNA length standard VI: 154, 220/234, 298, 394, 453, 517, 653, 1033 bp, Boehringer Mannheim.)