Intracellular localization of pyrin.

COS-1 cells were transiently transfected with a vector expressing either EGFP or EGFP-pyrin. (A) Western blot analysis of transfected cells (lane 1: EGFP-pyrin, lane 2: EGFP vector control) using a monoclonal anti-GFP antibody. (B) COS-1 cells either transfected with an EGFP-pyrin or an EGFP expression vector were grown on cover slips and analyzed by fluorescent microscopy (1000 × magnification).