Fig. 2.
![Fig. 2. Stat6 decoy inhibits mRNA expression of IL-4 but not other cytokines. / RNA for RPA analysis was obtained from untreated control samples (lanes a, b, g), Stat6 decoy (lane c), scrambled (lane d) ODN-transfected D10 cells, or cells pretreated with anti–IL-3 or anti–IL-4 antibodies (lanes e and f, respectively). Cells were stimulated with IL-4 (lanes b through d). RNA was isolated and hybridized with [33P]-labeled RNA probes corresponding to transcripts for murine cytokines (mCK1). The RNase-protected fragments were separated on 5% PAGE, dried, and exposed to x-ray. Densitometric analysis of IL-4 RNA message compared with L32/GAPDH indicated a greater than 90% reduction in Stat6 decoy–treated samples.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/95/4/10.1182_blood.v95.4.1249.004k39_1249_1257/6/bloo00439002w.jpeg?Expires=1735507936&Signature=B8PifdWrLRaY1xF7AcCaRWldr1p-zO-~sPkLXRiTQhp0Xqh7DbIVfCoOndhu2ufgVR03KVy7A2qsEnDD6nuD9YErkcvarYJl7NQ-XQgYQOHUPk7D7L~txw8tm4XJPhsqbWLKoAJmTcPetaXy8PGWhAENTnUdCDE4V2Xrq1VcFTmctMauzHhJ5-3ZrTzB1vrY1Rhd8LLQQBHQXrQ2mC43070lvreSX4WaydcSY-yVrlYnZ8ozlu4-xIRU5mhios2itxgiTWY10JGhIUo0hsWrQdM1DPwtddNYxx2imWgzhVXXUgXclzKFteLFa5~PcQPWi3JA9f2dZN3Lee7VRRaqBw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Stat6 decoy inhibits mRNA expression of IL-4 but not other cytokines.
RNA for RPA analysis was obtained from untreated control samples (lanes a, b, g), Stat6 decoy (lane c), scrambled (lane d) ODN-transfected D10 cells, or cells pretreated with anti–IL-3 or anti–IL-4 antibodies (lanes e and f, respectively). Cells were stimulated with IL-4 (lanes b through d). RNA was isolated and hybridized with [33P]-labeled RNA probes corresponding to transcripts for murine cytokines (mCK1). The RNase-protected fragments were separated on 5% PAGE, dried, and exposed to x-ray. Densitometric analysis of IL-4 RNA message compared with L32/GAPDH indicated a greater than 90% reduction in Stat6 decoy–treated samples.
