Stat6 decoy treatment of TH2 clone D10 cells preferentially inhibits IL-4 production.
D10 cells were transfected with Stat6 decoy or scrambled ODN at 37°C for 6 hours, then resuspended and cultured in normal D10 medium for 24 hours. ELISA was performed for each cytokine indicated (n = 3) and plotted as the mean ± the standard error (abscissa) based on ELISA values (pg/mL) (ordinate). Actual values obtained were as follows: For control cells, IL-4 (4980 ± 518), IL-5 (7646 ± 511), IL-6 (5904 ± 518), IL-10 (8860 ± 240), and IL-13, (9926 ± 516). For Stat6 decoy ODN–transfected D10 cells, IL-4 (1461 ± 264), IL-5 (7448 ± 760), IL-6 (4906 ± 423), IL-10 (8479 ± 808), and IL-13 (8233 ± 431). For scrambled decoy ODN–transfected D10 cells, IL-4 (4308 ± 339), IL-5 (7260 ± 609), IL-6 (5276 ± 366), IL-10 (7972 ± 448), and IL-13 (10 996 ± 947).
