Fig. 4.
![Fig. 4. Stat6 decoy inhibits IL-4–inducible cell proliferation on a TH2 cell line and human CD4+ T cells. / Quiescent D10 cells (upper and middle panel) or human CD4+T cells (lower panel) (50 × 103/well) were transfected with Stat6 decoy or scrambled decoy ODN for 6 hours and cultured in 200 μL of growth media in the presence of IL-4 (▪, upper and lower panel) or IL-2 (▪, middle panel) or their absence (▨) for 16 hours at 37°C. Cells were then pulsed with [3H]-thymidine (0.5 μCi/200 μL) for an additional 4 hours and incorporation of radiolabeled probe plotted on the abscissa (expressed as total cpm for 6 samples).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/95/4/10.1182_blood.v95.4.1249.004k39_1249_1257/6/bloo00439004x.jpeg?Expires=1735830047&Signature=I8QIhbhSoq3Mpwqe-rkOzfv8A0iFmueD19Wi-f3xWAqzQFNbobTPvy436lToZpySYhwzwXoUD69YS1LnVz2Od3gIR3uyqNzq8kPn3RJMUMY0GVXsphEPYDk3KkvquSalOGHKjpYeh31oFHAq6miq0Rr5yL8~-DBnET7yDOvhLnkrvaaRn4MfDr1L~VDSdQqHf6QgqkmpbWXDgRKXE~n02Hj3A3w6HuUASKFcNrdwimmL~BZqwYqMzvfjGBtL1aTEm3RCXWX40AVk~3ViGibtloDis7~RTeCuW3ozvSaEohMUqyDFxn5OyyMGbiSMV3UvbO-gv8~3kZ2UGZX24K1jZQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Stat6 decoy inhibits IL-4–inducible cell proliferation on a TH2 cell line and human CD4+ T cells.
Quiescent D10 cells (upper and middle panel) or human CD4+T cells (lower panel) (50 × 103/well) were transfected with Stat6 decoy or scrambled decoy ODN for 6 hours and cultured in 200 μL of growth media in the presence of IL-4 (▪, upper and lower panel) or IL-2 (▪, middle panel) or their absence (▨) for 16 hours at 37°C. Cells were then pulsed with [3H]-thymidine (0.5 μCi/200 μL) for an additional 4 hours and incorporation of radiolabeled probe plotted on the abscissa (expressed as total cpm for 6 samples).
