Fig. 5.
![Fig. 5. Stat6 decoy does not alter mRNA expression of IL-4 receptors. / Freshly isolated mRNA was obtained from control (lanes a, b, g), Stat6 decoy (lane c), scramble ODN-transfected D10 cells (lane d), or anti–IL-4 antibody treated (lane f) or anti–IL-3 antibody treated (lane e) cells in the absence (lanes a, e, f) or presence of IL-4 (lanes b through d). RNA was then hybridized with [33P]-labeled RNA probes corresponding to transcripts for individual murine γc-receptors (mCR-1) according to PharMingen protocol (see “Materials and methods”). The Rnase-protected fragments were separated on 5% PAGE, dried, and exposed to x-ray film.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/95/4/10.1182_blood.v95.4.1249.004k39_1249_1257/6/bloo00439005w.jpeg?Expires=1742857902&Signature=aCg9Zd02eY88lKJSweOiS1d3L307tn6X2Il4mr4~MjBuMvv58U5ZeUXaQ3M3ub4u4A6VmENGOs9MqsjUrQ77xITYi7HuFlaj5gcEbBvknQNDrNkFSTp0OWZ~ZJYvK80wEzM~RNHkHj9rlvmedAFtWolnUxA0haax8x54hkOgojfWdQL909z-aulpUFtzhp8NKOnhDmZ3PQJkqrSXJPOFvZjDRCU7jktxj54mRgKOL0Oxi3Zpcc0dlRuL8o0vAUrzeJ4W7LBXi4~2-y-KEoqzAM~pE94H3xBTYdnxeZ4uisLVFaBQPKSll2r4briCoJ39bEqYeNvehjK~BNhp3hNk0Q__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Stat6 decoy does not alter mRNA expression of IL-4 receptors.
Freshly isolated mRNA was obtained from control (lanes a, b, g), Stat6 decoy (lane c), scramble ODN-transfected D10 cells (lane d), or anti–IL-4 antibody treated (lane f) or anti–IL-3 antibody treated (lane e) cells in the absence (lanes a, e, f) or presence of IL-4 (lanes b through d). RNA was then hybridized with [33P]-labeled RNA probes corresponding to transcripts for individual murine γc-receptors (mCR-1) according to PharMingen protocol (see “Materials and methods”). The Rnase-protected fragments were separated on 5% PAGE, dried, and exposed to x-ray film.
