Stat6 decoy does not affect Stat6 tyrosine phosphorylation.

D10 cells were treated with Stat6 decoy (lanes c, d) or scrambled ODN (lanes e, f) for 6 hours and then stimulated with or without 100 nmol/L IL-4 at 37°C for 10 minutes. Cells were lysed, immunoprecipitated with anti-Stat6 (α-Stat6), and then Western blotted with αPY (upper panel) or anti-Stat6 (lower panel) to verify equivalent loading. Arrows indicate location of Stat6.