Fig. 9.
Fig. 9. Effects of Stat6 decoy ODN on transactivation of Stat6 compared with Stat1/3 in cultured D10 cells. / (A) D10 cells treated with Stat6 decoy ODN or scrambled decoy ODN were co-transfected with a 3 × Stat6 binding element pGL3 promoter–luciferase construct or with pGL3 promoter–luciferase construct alone. After transfection, cells were stimulated with or without IL-4 (100 nmol/L). Cells were harvested after an additional 24 hours, and luciferase activity was measured and normalized against protein concentration. (B) D10 cells treated with Stat6 decoy ODN, or scrambled decoy ODN were co-transfected with a 3 × SIE binding element pGL3 promoter–luciferase construct or with pGL3 promoter–luciferase construct alone. After transfection, cells were stimulated with or without IL-2 (100 nmol/L). Cells were harvested after an additional 24 hours, and luciferase activity was measured and normalized against protein concentration.

Effects of Stat6 decoy ODN on transactivation of Stat6 compared with Stat1/3 in cultured D10 cells.

(A) D10 cells treated with Stat6 decoy ODN or scrambled decoy ODN were co-transfected with a 3 × Stat6 binding element pGL3 promoter–luciferase construct or with pGL3 promoter–luciferase construct alone. After transfection, cells were stimulated with or without IL-4 (100 nmol/L). Cells were harvested after an additional 24 hours, and luciferase activity was measured and normalized against protein concentration. (B) D10 cells treated with Stat6 decoy ODN, or scrambled decoy ODN were co-transfected with a 3 × SIE binding element pGL3 promoter–luciferase construct or with pGL3 promoter–luciferase construct alone. After transfection, cells were stimulated with or without IL-2 (100 nmol/L). Cells were harvested after an additional 24 hours, and luciferase activity was measured and normalized against protein concentration.

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