Expression of fibroblast growth factor receptor 3 (FGFR3) is achieved at variable levels in B9-WT and B9-TD cells.

(A) Western blot analysis, probing with anti-FGFR3, shows the expression of FGFR3 in B9 clones: KMS11 myeloma cell line positive control for FGFR3 (lane 1), parental B9 (lane 2), and B9-WT subclones (lanes 3-15). Lane 4 (clone #3) and lane 7 (clone #6) represent clones selected for further study. (B) KMS11 positive control for FGFR3 (lane 1), U266 myeloma cell line negative control (lane 2), parental B9 (lane 3), and B9-TD subclones (lanes 4-18). Lane 17 represents clone #14 selected for further study.