STAT3 phosphorylation in fibroblast growth factor receptor 3 (FGFR3)-expressing cells.

FGFR3-expressing and control cell lines were depleted of cytokine and then stimulated with or without interleukin-6 for 10 minutes at 37°C. B9-WT clones selected for high expression of FGFR3 were used. Total cell lysates were resolved via SDS-PAGE, and the membrane was probed with a tyrosine phospho-specific STAT3 antibody (upper panel). The membrane was reprobed for loading equivalence with a peptide-specific antibody that recognized total STAT3 (lower panel).