Cell dose-dependent engraftment of CD34+ PBPC in NOD/SCID mice (n = 63) after injection of Transwell-transduced or mock-transduced cells under various culture conditions.
The number of transplanted cells reflects the input CD34+ cell number (ie, after transduction, the cells were not recounted before xenotransplantation). The proportions of human CD45+ leukocytes in bone marrow (
), spleen (□), and peripheral blood (▪) determined by FACS are shown as mean ± SE values. In each experiment, normal human PB and mice not given transplants were analyzed as controls. Comparison of human-cell engraftment between mice injected with 5 × 105 CD34+ cells after retroviral transduction in the presence of IL-3 or IL-3/FL yieldedP = .01 (**). The 3 denotes IL-3; 6, IL-6; FL, Flt3-ligand; T, thrombopoietin; S, stem-cell factor; and V, vascular epithelial growth factor.
