Examples of the analysis of chromocenters in lymphocytes and fibroblasts using confocal microscopy.

(A) Optical series of a lymphocyte nucleus hybridized with the pan-centromeric probe. A reduced number of hybridization signals is observed, corresponding to the coalescence of signals originated by individual centromeres. Note that most of the signals are visible in several sequential optical sections. (B) Analysis of chromocenters in quiescent human fibroblasts. Optical sections obtained in different nuclei. Bars, 10 μm. The nuclear lamina is labeled with an anti-laminin B antibody.